Ginger honey affects cortisol, estrogen and glutathione levels; preliminary study to target preconceptional women.

OBJECTIVE: This study was aimed to determine the effect of ginger honey supplementation on cortisol, glutathione, and estrogen levels. The study was conducted on mice that had not yet experienced conception, and prior stress induction was carried out so that they could be continued for human trials at the preconception stage and subjects who experienced mild stress. METHOD: It was an in vivo study, pretest-posttest control group design. The sample of this study was 2-3 months female Balb/c mice, divided into negative control and ginger honey intervention as much as 28mg/20g BW for 14 days-the ELISA method used to examine cortisol hormone, glutathione levels, and estrogen levels. The mice chosen were those that had never experienced conception, and before the intervention, swimming activities were carried out on the mice until they showed symptoms of stress. RESULTS: Results show 42mg/20g BW of ginger honey administration for 14 days increased 1.892 ng/dl of cortisol (p = 0.165), increased 2.438 ng/dl of glutathione (p=0.002), and also increased 22.754ng/ml estrogen levels in induced stress Balb/c female mice (p=0.001). CONCLUSION: Ginger honey did not affect reducing cortisol levels but increasing glutathione and estrogen levels significantly. Ginger honey supplements are the potential to use as complementary therapies.

Analysis of cocktail honey content as a supplementary preparation in preconception women.

OBJECTIVE: This study is basic research carried out to measure the nutritional content of the honey cocktail product, an herbal therapy as a complementary treatment in addressing reproductive health problems and improving the nutritional status of preconception women. The purpose of this research is to know the ingredients in honey cocktail products. METHOD: The stage of this study is; choose a sample, processing honey cocktail, and testing the parameters. This study used three main raw materials, namely honey, royal jelly, and bee bread. The honey used is Trigona sp. The three primary raw materials used have been processed from the Halal Center of Hasanuddin University. Honey cocktail processing will use a mixture of 100g Trigona sp. honey, 100g royal jelly, and 100g bee bread, then be homogenized using a magnetic stirrer for ±15min. Honey quality testing is conducted to determine the nutritional content of the honey cocktail supplement, such as vitamins, minerals, proteins, fats, carbohydrates, sugars (sucrose and glucose), hydroxymethylfurfural, and metals, and water. This study also examined the metal contamination, ash, and fat level. RESULTS: The results of the test content in the cocktail honey supplement found in 100ml containing 61.9g carbohydrates, 0.7g of protein, 2.3mg of phosphorus, 0.3mg of iron, 1736.27µg/g of potassium, as much calcium 48.35µg/g, 0.2mg of manganese, 0.04mg of fat content, and 2.84% of vitamin A. in addition, cocktail honey also contains 3.8% sucrose, 78% glucose, HMF 48.63mg/kg, Cu <0.01µg/g, PB 0.05µg/g, arsenic <0.01, acidity 49.62ml NaOH/kg, an ash content of 0.2%, and moisture content of 18.24%. CONCLUSION: The conclusion in this study cocktail honey samples meets the honey quality requirements following the Indonesian national standard. So that the cocktail honey supplement has good quality and is safe for consumption.

Effect of royal jelly to deal with stress oxidative in preconception women: A literature review.

INTRODUCTION: Oxidative stress that occurs in preconception women can disrupt the reproductive system to cause infertility. The antioxidants contained in royal jelly can overcome oxidative stress due to low antioxidants in the body. The purpose of this study is to examine studies of the content of royal jelly, antioxidant activity, and the effectiveness of royal jelly in dealing with oxidative stress in preconception women. METHOD: This research method is an electronic database search using keywords according to questions in research from the online library PubMed, content science, and Science Direct. RESULT: a study review conducted in 6 research journals stated that the use of royal jelly as a supplement containing 10-hydroxy-2-decanoic (10-HDA) increases glutathione levels, as well as lipid peroxidation inhibitors. CONCLUSION: The effectiveness of royal jelly to overcome oxidative stress in preconception women can be assessed from the content of royal jelly and antioxidant activity that can increase the glutathione levels and inhibit increased lipid peroxidation, which is a sign of oxidative stress.

Ginger honey affects cortisol, estrogen and glutathione levels; preliminary study to target preconceptional women

Objective: This study was aimed to determine the effect of ginger honey supplementation on cortisol, glutathione, and estrogen levels. The study was conducted on mice that had not yet experienced conception, and prior stress induction was carried out so that they could be continued for human trials at the preconception stage and subjects who experienced mild stress. Method: It was an in vivo study, pretest–posttest control group design. The sample of this study was 2–3 months female Balb/c mice, divided into negative control and ginger honey intervention as much as 28 mg/20 g BW for 14 days—the ELISA method used to examine cortisol hormone, glutathione levels, and estrogen levels. The mice chosen were those that had never experienced conception, and before the intervention, swimming activities were carried out on the mice until they showed symptoms of stress. Results: Results show 42 mg/20 g BW of ginger honey administration for 14 days increased 1.892 ng/dl of cortisol (p = 0.165), increased 2.438 ng/dl of glutathione (p = 0.002), and also increased 22.754 ng/ml estrogen levels in induced stress Balb/c female mice (p = 0.001). Conclusion: Ginger honey did not affect reducing cortisol levels but increasing glutathione and estrogen levels significantly. Ginger honey supplements are the potential to use as complementary therapies. (AU)

Effect of royal jelly to deal with stress oxidative in preconception women: A literature review

Introduction: Oxidative stress that occurs in preconception women can disrupt the reproductive system to cause infertility. The antioxidants contained in royal jelly can overcome oxidative stress due to low antioxidants in the body. The purpose of this study is to examine studies of the content of royal jelly, antioxidant activity, and the effectiveness of royal jelly in dealing with oxidative stress in preconception women. Method: This research method is an electronic database search using keywords according to questions in research from the online library PubMed, content science, and Science Direct. Result: a study review conducted in 6 research journals stated that the use of royal jelly as a supplement containing 10-hydroxy-2-decanoic (10-HDA) increases glutathione levels, as well as lipid peroxidation inhibitors. Conclusion: The effectiveness of royal jelly to overcome oxidative stress in preconception women can be assessed from the content of royal jelly and antioxidant activity that can increase the glutathione levels and inhibit increased lipid peroxidation, which is a sign of oxidative stress. (AU)

Analysis of cocktail honey content as a supplementary preparation in preconception women

Objective: This study is basic research carried out to measure the nutritional content of the honey cocktail product, an herbal therapy as a complementary treatment in addressing reproductive health problems and improving the nutritional status of preconception women. The purpose of this research is to know the ingredients in honey cocktail products. Method: The stage of this study is; choose a sample, processing honey cocktail, and testing the parameters. This study used three main raw materials, namely honey, royal jelly, and bee bread. The honey used is Trigona sp. The three primary raw materials used have been processed from the Halal Center of Hasanuddin University. Honey cocktail processing will use a mixture of 100 g Trigona sp. honey, 100 g royal jelly, and 100 g bee bread, then be homogenized using a magnetic stirrer for ±15 min. Honey quality testing is conducted to determine the nutritional content of the honey cocktail supplement, such as vitamins, minerals, proteins, fats, carbohydrates, sugars (sucrose and glucose), hydroxymethylfurfural, and metals, and water. This study also examined the metal contamination, ash, and fat level. Results: The results of the test content in the cocktail honey supplement found in 100 ml containing 61.9 g carbohydrates, 0.7 g of protein, 2.3 mg of phosphorus, 0.3 mg of iron, 1736.27 μg/g of potassium, as much calcium 48.35 μg/g, 0.2 mg of manganese, 0.04 mg of fat content, and 2.84% of vitamin A. in addition, cocktail honey also contains 3.8% sucrose, 78% glucose, HMF 48.63 mg/kg, Cu <0.01 μg/g, PB 0.05 μg/g, arsenic <0.01, acidity 49.62 ml NaOH/kg, an ash content of 0.2%, and moisture content of 18.24%. Conclusion: The conclusion in this study cocktail honey samples meets the honey quality requirements following the Indonesian national standard. So that the cocktail honey supplement has good quality and is safe for consumption. (AU)

Interleukin-6 expression on inflamed rat dental pulp tissue after capped with Trigona sp. propolis from south Sulawesi, Indonesia.

Background: Propolis is a natural product of plant resins collected by honeybees from various plant sources. It is used as a remedy in folk medicine since ancient times because of its several biological and pharmacological properties. Recently, propolis has been used by dentist to treat various oral diseases. It was always mentioned as an anti-inflammatory agent. Cytokines are proteins that provide communication between cells and play a critical role in a wide variety of processes. It released from cells in an inflammatory process that active, mediate or potential actions of other cells or tissues. When dental pulp has inflammation, several pro-inflammatory cytokines including Interleukin-6 (IL-6) was released by innate immune cells. Objective: To analyse the expression of IL-6 on inflamed rat dental pulp tissue following application of propolis. Material and methods: Trigona sp. propolis was obtained from Luwu Regency, south Sulawesi Province, Indonesia. Flavonoid and non-flavonoid extracts were purified from propolis using thin layer chromatography. The study was applied on 80 male Sprague Dawley rats, 10-12 weeks of age, divided randomly and equally into 5 groups. Group I, as negative control group was not conducted any treatment. At group II, III, IV and V. A Class I cavity (Black Classification) were made on the occlusal surface of right maxillary first molar. The dental pulp was perforated using dental explorer and allowed in the oral environment for 1 h, after that, Ethanolic Extract Propolis (EEP) (Group II), Extract Flavonoid-Propolis (EFP) (Group III), Extract Non-Flavonoid Propolis (ENFP) (Group IV), or Calcium Hydroxide (Ca(OH)2) (Group V) were applied on dental pulp. All cavities were then filled with Glass Ionomer Cement as permanent filling. The rats being sacrificed in 6 h, 2 days, 4 days and 7 days. Sample biopsy were obtained, IL-6 expression was detected by using immunohistochemistry method. Data was analyzed statistically using Freidman and Kruskal Wallis tests with significance level of P < 0.05. Results: All agent showed IL-6 expression in inflamed rat dental pulp tissue, and this expression was decreased with the longer of observation time periods. EEP more stronger to decreased IL-6 expression on inflamed rat dental pulp tissue than other agent. There is significant difference (P < 0.05) of IL-6 expression between group I and other groups in 6 h and 2 days but not in 4 and 7 days time periods. Conclusion: Trigona sp. propolis from south Sulawesi, Indonesia could suppressed the expression of IL-6 on inflamed rat dental pulp tissue.